Ambesse Tolosa (born 28 August 1977 in Shewa) is an Ethiopian long-distance runner, who specializes in the marathon race.

Tolosa tested positive for banned substances in February 2008 and received a two-year ban from competitive athletics. The substance that was present in his system (morphine) was not a performance-enhancing drug and Tolosa said that he did not know how it had gotten into his body, but the IAAF rules stated that athletes received bans regardless of intent.^[1] His results from 9 December 2007 onwards were annulled,^[2] which included his win at the Honolulu Marathon that year.

• All information taken from IAAF profile.