Charlotte of Nassau-Dillenburg-Schaumburg of Anhalt-Bernburg-Hoym (1672–1700) was the wife of Lebrecht, Prince of Anhalt-Zeitz-Hoym, a German prince of the House of Ascania.

Charlotte of Nassau-Dillenburg-Schaumburg was born on 25 September 1672 in Northrhine-Westphalia, Germany. She was heiress to the County of Holzappel and Lordship of Schaumburg and the youngest of three surviving daughters of Adolph, Prince of Nassau-Schaumburg. Charlotte was declared the sole heiress of the County of Holzappel (which included the towns of Holzappel and Charlottenberg) and the Lordship of Schaumburg.^[1]

By a contract made on 1 September 1690 between Victor Amadeus, Prince of Anhalt-Bernburg and Elisabeth Charlotte Melander, Countess of Holzappel, Charlotte was betrothed to Lebrecht, Prince of Anhalt-Zeitz-Hoym The marriage was performed in Schaumburg on 12 April 1692. They had five children:

• Victor I Amadeus Adolph, Prince of Anhalt-Bernburg-Schaumburg-Hoym (Schaumburg, 7 September 1693 – Schaumburg, 15 April 1772).
• Frederick William (Schaumburg, 12 April 1695 – killed in action at Denain, 24 July 1712).
• Elisabeth Charlotte (Bernburg, 4 December 1696 – Schaumburg, 14 June 1754).
• Christian (Bernburg, 27 November 1698 – killed in action at Palermo, 29 April 1720).
• Victoria Hedwig (Bernburg, 30 January 1700 – Schaumburg, 10 February 1701).

She died, aged 27, on 31 January 1700, and is interred in the church of Saint Aegidien in Bernburg, Germany.