Lipid_IVA_4-amino-4-deoxy-L-arabinosyltransferase

Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase

Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase

Class of enzymes

Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase (EC 2.4.2.43, undecaprenyl phosphate-alpha-L-Ara4N transferase, 4-amino-4-deoxy-L-arabinose lipid A transferase, polymyxin resistance protein PmrK, arnT (gene)) is an enzyme with systematic name 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans, octacis-undecaprenyl phosphate:lipid IVA 4-amino-4-deoxy-L-arabinopyranosyltransferase.[1][2][3][4][5] This enzyme catalyses the following chemical reaction

(1) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans, octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans, octacis-undecaprenyl phosphate
(2) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans, octacis-undecaprenyl phosphate + lipid IVA lipid IIA + ditrans, octacis-undecaprenyl phosphate
(3) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans, octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA 4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + ditrans, octacis-undecaprenyl phosphate
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This integral membrane protein is present in the inner membrane of certain Gram negative endobacteria.

References

  1. [1]
    Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR (November 2001). "An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor". The Journal of Biological Chemistry. 276 (46): 43122–31. doi:10.1074/jbc.m106961200. PMID 11535604.
  2. [2]
    Trent MS, Ribeiro AA, Doerrler WT, Lin S, Cotter RJ, Raetz CR (November 2001). "Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm". The Journal of Biological Chemistry. 276 (46): 43132–44. doi:10.1074/jbc.m106962200. PMID 11535605.
  3. [3]
    Zhou Z, Ribeiro AA, Lin S, Cotter RJ, Miller SI, Raetz CR (November 2001). "Lipid A modifications in polymyxin-resistant Salmonella typhimurium: PMRA-dependent 4-amino-4-deoxy-L-arabinose, and phosphoethanolamine incorporation". The Journal of Biological Chemistry. 276 (46): 43111–21. doi:10.1074/jbc.m106960200. PMID 11535603.
  4. [4]
    Bretscher LE, Morrell MT, Funk AL, Klug CS (March 2006). "Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium". Protein Expression and Purification. 46 (1): 33–9. doi:10.1016/j.pep.2005.08.028. PMID 16226890.
  5. [5]
    Impellitteri NA, Merten JA, Bretscher LE, Klug CS (January 2010). "Identification of a functionally important loop in Salmonella typhimurium ArnT". Biochemistry. 49 (1): 29–35. doi:10.1021/bi901572f. PMC 2805049. PMID 19947657.
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