This is a list of intersex characters in fiction, i.e. fictional characters that either self-identify as intersex or have been identified by outside parties to be intersex. Listed characters are either recurring characters, cameos, guest stars, or one-off characters.

For more information about fictional characters in other parts of the LGBTQ community, see the lists of lesbian (with sub-pages for characters in anime and animation), bisexual (with sub-sections for characters in anime and animation), gay, non-binary, trans, pansexual, and asexual characters.

The names are organized alphabetically by surname (i.e. last name), or by single name if the character does not have a surname. If more than two characters are in one entry, the last name of the first character is used.

• Films about intersex
• Literature about intersex
• Television works about intersex
• List of intersex people
• List of fictional polyamorous characters
• List of animated series with LGBT characters
• Lists of LGBT figures in fiction and myth
• List of animated series with crossdressing characters