The following is a list of presently operating intercity bus stops in Iowa with regular service. The list excludes charter buses, local transit buses, paratransit systems, and trolleybus systems. The following companies provide intercity bus service in Iowa as of September 2023:

• Burlington Trailways
• Greyhound Lines
• Jefferson Lines
• Lamers Bus Lines
• Tornado Bus Company

This is the list of 32 active intercity bus stops serving 28 cities in Iowa. This list does not include stops that are served only by commuter buses and not intercity buses. This list also does not include Tornado Bus Company stops, due to the difficulty of acquiring information on routes and stop locations.^[1][2][3][4]

The following intercity bus stops in Illinois and Nebraska connect with local transit systems which operate in Iowa.^[5]

• List of Amtrak stations
• List of intercity bus stops in:
  • Illinois
  • Minnesota
  • Missouri
  • Nebraska
  • South Dakota
  • Wisconsin