Mary Frances Lyon FRS^[4] (15 May 1925 – 25 December 2014)^[5] was an English geneticist best known for her discovery of X-chromosome inactivation, an important biological phenomenon.^{[6][7][8][9][10][11][12]}

Mary Lyon was born on 15 May 1925 in Norwich, England as the eldest out of three children of a civil servant and a schoolteacher. She was educated at a grammar school in Birmingham. During that time, she said, she became interested in science thanks to a good schoolteacher^[13] and nature books she won in an essay competition.^[14] During the Second World War in 1943, she began her studies at Girton College, Cambridge at the University of Cambridge,^[15] where she read zoology, physiology, organic chemistry and biochemistry, with zoology as her main subject. At this time, only 500 (less than 10%) female students were allowed to study at the university, in contrast to more than 5,000 men. Furthermore, despite doing the same work as male students, female students received only “titular" degrees, rather than full Cambridge degrees that would make them members of the university.^[14] During her studies at Cambridge, she became interested in embryology. She went on to do her PhD with Ronald Fisher, who was Professor of Genetics in Cambridge, where she characterised a mutant mice strain with a 'pallid' mutation and published the research. During the course of her PhD she moved to the University of Edinburgh, where she completed her studies under the direction of Douglas Falconer.^[16]

After her PhD (awarded 1950^[17]), Lyon joined the group of Conrad Hal Waddington, with whom she worked in the last part of her PhD. The group was funded by the Medical Research Council, and she worked with TC Carter to investigate mutagenesis and the genetic risks of radiation.^[16] In addition to the 'pallid' mutation mice, she studied mutations such as 'ataxia' (a nervous mutation which caused walking difficulties in the mice) and 'twirler' (a mutation which induced inner ear issues, causing the mice to shake their heads and walk in circles due to lack of balance).

In 1955, her group moved to the MRC radiobiology unit in Harwell, where there was room for more mouse facilities. There she continued to investigate the mouse mutations. She also scrutinised a 'mottled' mutant, which had a different effect on male and female mice: male embryos sometimes died, and the surviving males had white coats, but females lived and were variegated. Through calculated and deliberated breeding of mutants, she investigated the transition of the mutation and concluded that the mutation was positioned on the X chromosome. This, together with new findings at that time concerning the X chromosome, led her to hypothesize about X chromosome silencing.^[14]

Lyon published many papers on radiation and chemical mutagenesis and on studies of mutant genes.^[18] She also did extensive work on the mouse t-complex.^[19][20]

She was head of the Genetics Section of the MRC Radiology Unit at Harwell from 1962 to 1987. Although she retired from research in 1990, according to an interview from 2010, she was still active in the laboratory a few times a week.^[14]