Mexborough Athletic F.C. was an English association football club based in Mexborough, Doncaster, South Yorkshire.

The club was formed as Mexborough Town in 1903, and was the second team to take the Mexborough name, the first having been wound up three years earlier.^[1][2]

They spent their first season in the Sheffield Association League, winning the league title at the first attempt. The club joined the Midland League in 1905 and would remain in the competition for the rest of their existence.

The Midland League was suspended for the duration of the First World War, but Mexborough rejoined the competition when hostilities ended. The club won its only Midland League title in 1926, two years after changing their name to Mexborough Athletic.^[3] The following year they reached the first round of the FA Cup, losing to Chesterfield at Saltergate.

Athletic would struggle on in the lower echelons of the Midland League until 1936, when after finishing bottom of the table, they resigned from the competition and disbanded.^[4]

• Best FA Cup performance: 1st round, 1927–28