Image | Name | Born | Died | Position | Additional information |
| Sir Ernest Shackleton | 1874 | 1922 | Commander | Died on South Georgia during the expedition |
| Frank Wild | 1873 | 1939 | 2nd in Command | Took command of the expedition on Shackleton's death. Had served as 2nd in Command on Shackleton's Imperial Trans-Antarctic Expedition |
| Frank Worsley | 1872 | 1943 | Captain of Quest | Formerly Captain of Endurance during the Imperial Trans-Antarctic Expedition |
| Alexander Kerr | 1892 | 1964 | Engineer | Served as 2nd Engineer on the Imperial Trans-Antarctic Expedition |
| James McIlroy | 1879 | 1968 | Surgeon/Meteorologist | Veteran of the Imperial Trans-Antarctic Expedition |
| Alexander Macklin | 1889 | 1967 | Surgeon/In charge of stores and equipment | Veteran of the Imperial Trans-Antarctic Expedition |
| Leonard Hussey | 1891 | 1964 | Meteorologist/Assistant Surgeon | Accompanied Shackleton's body to Montevideo and then back to South Georgia, so was not present for most of the expedition. Veteran of the Imperial Trans-Antarctic Expedition |
| Charles Green | 1888 | 1974 | Cook | Veteran of the Imperial Trans-Antarctic Expedition |
| Thomas McLeod | 1869 | 1960 | Able seaman | Veteran of the Imperial Trans-Antarctic Expedition |
| Roderick Carr | 1891 | 1971 | Pilot | Assisted with scientific work as the expedition's plane was unserviceable |
| James Dell | c.1880 | 1968 | Electrician, Boatswain | Had been with Shackleton on the Discovery Expedition |
| Hubert Wilkins | 1888 | 1958 | Biologist | |
| George Vibert Douglas | 1892 | 1958 | Geologist | Born in Montreal, Canada. Professor of Geology, Dalhousie University (1932-1957) |
| James Marr | 1902 | 1965 | Boy Scout | With Mooney was selected from 1,700 Scouts who applied to accompany the expedition |
| Norman Mooney[1] | c.1904 | | Boy Scout | Left the ship at Madeira after suffering chronic seasickness |
| Douglas George Jeffrey | 1885 | 1972 | Navigator | Had been intended for the original crew on the Imperial Trans-Antarctic Expedition, but when war broke out on the eve of Endurance's departure he felt compelled to stay in England to sign up. |
| Eriksen[2] | | | Harpoon expert | Returned home when the Quest put in at Rio de Janeiro for repairs |
| John Charles Bee-Mason[3] | c.1875 | 1957 | Photographer | Left the ship at Madeira after suffering chronic seasickness |
| C. E. Smith | | | 2nd Engineer | |
| Harold Watts | | | Wireless Operator | |
| G. H. Ross[4] | | | Stoker | |
| S. S. Young | | | Stoker | Joined the expedition in Rio de Janeiro |
| Harold Arthur Argles[5] | 1899 | 1929 | Stoker | Joined the expedition in Rio de Janeiro. |
| Christopher Naisbitt | | | Cook's assistant | Joined the expedition in Rio de Janeiro |
| Query (dog) | 1920/21[6] | 1922 | Mascot | Washed overboard and drowned on June 9, 1922 |
| Questie (cat) | 1921 | | Mascot | A kitten presented to the expedition by the Daily Mail |