Carlos Rodolfo Ambrosio was born on 27 December 1961 in Córdoba, Argentina.He is an Argentine rugby union former player where he played as a fly-half and as a center.

He played for Tala Rugby Club in Córdoba, Argentina. He was the first Italian Argentine to play for the Italy national rugby union team. He had 12 caps for Italy from 1987 to 1989 where he scored 1 try and 3 penalties, 13 points on aggregate. He was called for the 1987 Rugby World Cup. He played a single game on 22 May 1987 in what was his debut for the Italian side versus New Zealand, in Auckland at a 70-6 points and he didn't score at his single presence in the competition. Ambrosio's last game was in Moscow on 5 November 1989 at a 15-12 loss to the Soviet Union, for the 1989-1990 FIRA Trophy.

He returned to Argentina after finishing his player career where he was head coach of Córdoba Rugby Club from 1999 to 2000. He came back to Italy where he was a head coach of Petrarca Rugby from 2002/03 to 2003/04 and Segni from 2005/06 to 2006/07. In October 2008 to 2011, he was the director of the Federal Center of Development and High Performance of the province of Córdoba. After his replacement by Francisco Rubio, he took over the direction of the federal technical sector of the youth.

He was the head coach of Argentina national under-20 rugby union team at the 2014 IRB Junior World Championship, finishing in 9th place.

In November 2014, he was announced as the new head coach of the Brazil national rugby union team. He was in charge until 2019.^[1]

In 2024 he was appointed head coach of the Uruguay national rugby union team.^[2]