The Screen Actors Guild Award for Outstanding Performance by a Female Actor in a Supporting Role in a Motion Picture is an award presented annually by the Screen Actors Guild. It has been presented since the 1st Screen Actors Guild Awards in 1995 to a female actor who has delivered an outstanding performance in a supporting role in a film released that year.

The award has been presented 30 times, with 30 actresses winning the award. Dianne Wiest was the award's first winner for Bullets over Broadway (1994). The most recent winner is Da'Vine Joy Randolph who won for her performance in The Holdovers (2023). Kate Winslet has won the award twice; no other actress has won it more than once. Cate Blanchett has the most nominations with five, with one win. At the 4th Screen Actors Guild Awards, Kim Basinger and Gloria Stuart both received the award; the only time this category has been tied.

Two awards

• Kate Winslet (Sense and Sensibility (1995), The Reader (2008))

Note: Winners are indicated in bold type.

Two nominations

• Kathy Bates (Primary Colors (1998), About Schmidt (2002))
• Emily Blunt (A Quiet Place (2018), Oppenheimer (2023))
• Hong Chau (Downsizing (2017), The Whale (2022))
• Jamie Lee Curtis (True Lies (1994), Everything Everywhere All at Once (2022))
• Viola Davis (Doubt (2008), Fences (2016))
• Cameron Diaz (Being John Malkovich (1999), Vanilla Sky (2001))
• Sally Field (Forrest Gump (1994), Lincoln (2012))
• Holly Hunter (Thirteen (2003), The Big Sick (2017))
• Frances McDormand (Almost Famous (2000), North Country (2005))
• Helen Mirren (Gosford Park (2001), Trumbo (2015))
• Margot Robbie (Mary Queen of Scots (2018), Bombshell (2019))
• Octavia Spencer (The Help (2011), Hidden Figures (2016))
• Emma Stone (Birdman (2014), The Favourite (2018))
• Rachel Weisz (The Constant Gardener (2005), The Favourite (2018))
• Michelle Williams (Brokeback Mountain (2005), Manchester by the Sea (2016))
• Renée Zellweger (Jerry Maguire (1996), Cold Mountain (2003))

Three nominations

• Penélope Cruz (Vicky Cristina Barcelona (2008), Nine (2009), Ferrari (2023))
• Judi Dench (Shakespeare in Love (1998), Chocolat (2000), The Shipping News (2001))
• Catherine Keener (Being John Malkovich (1999), Capote (2005), Into the Wild (2007))
• Nicole Kidman (The Paperboy (2012), Lion (2016), Bombshell (2019))
• Julianne Moore (Boogie Nights (1997), Magnolia (1999), The Hours (2002))

Four nominations

• Amy Adams (Junebug (2005), Doubt (2008), The Fighter (2010), Vice (2018))
• Kate Winslet (Sense and Sensibility (1995), Quills (2000), The Reader (2008), Steve Jobs (2015))

Five nominations

• Cate Blanchett (Bandits (2001), The Aviator (2004), Notes on a Scandal (2006), I'm Not There (2007), Nightmare Alley (2021))

• Academy Award for Best Supporting Actress
• BAFTA Award for Best Actress in a Supporting Role
• Independent Spirit Award for Best Supporting Female
• Critics' Choice Movie Award for Best Supporting Actress
• Golden Globe Award for Best Supporting Actress – Motion Picture