3D-animation_of_the_diatom_Corethron_sp.ogg
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Summary
Description 3D-animation of the diatom Corethron sp.ogv |
English:
3D-animation of a Diatom (Corethron sp.) which illustrates how the e-HCFM method supports investigation about microbial interactions
The specimen (surface water, Tara Ocean station 137) was imaged manually from regular e-HCFM sample preparation with a Leica SP8 confocal laser scanning microscope (40X NA1.1 water). Four fluorescent channels are recorded: (i) Green: cellular membranes (DiOC6(3)) indicate the core cell bodies; (ii) Blue: DNA (Hoechst) is used to identify the nucleus; (iii) Red: chlorophyll (autofluorescence); (iv) Grey: PLL-Alexa546 is a useful counterstain for visualizing the specimen surface. 3D reconstruction, surface rendering and animation were conducted with the software Imaris (Bitplane).
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Date | |
Source | doi : 10.7554/eLife.26066.018 |
Author | Sebastien Colin, Luis Pedro Coelho, Shinichi Sunagawa, Chris Bowler, Eric Karsenti, Peer Bork, Rainer Pepperkok, Colomban de Vargas |
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